Archive | Microbial Biodiversity

Effects of Bioenergy Agroecosystems on Soil Microbial Communities

There is growing societal interest in management of agricultural systems for bioenergy feedstock production, but further environmental evaluation of these systems is warranted. Measurement of soil microbial communities and activity may be useful in environmental evaluations of alternative bioenergy cropping systems, as they are important components of soil health. We characterized microbial community [...] More »

Effects of Agricultural Cropping Systems on Soil Microbial Community Structure

Microorganisms of the Bacteria (no nucleus), Eucaryotes (nucleus), and Archae (an ancient anaerobic group) were sampled using molecular techniques so that unculturable microorganisms could also be assessed. The general structure of the microbial community changed with cropping system (CS3, CS5, CS6) at Arlington in one soil sampling time in 1996. The Eukaryotes dominated [...] More »

Biodiversity of Pythium and Fusarium from Zea mays roots in different cropping systems

Certain species of fungi in the genera Pythium and Fusarium are pathogenic to corn (Zea mays). It is our hypothesis that isolation and measurement of these fungal agents from corn in field sites may lead to a better understanding of the relationship between crop rotation and pathogen build-up and action in the soil. [...] More »

Molecular Microbial Diversity of an Agricultural Soil in Wisconsin

A culture-independent survey of the soil microbial diversity in a clover/grass pasture in southern Wisconsin was conducted by sequence analysis of a universal small subunit rDNA clone library.  A rapid and efficient method for extraction of DNA from soils was developed which resulted in highly purified DNA with minimal shearing. Authors: J. Borneman, P. Skroch, K. [...] More »

Soil Microbial Biodiversity in Agroecosystems

Microbial populations are being analyzed by using rRNA probes in hybridization assays to evaluate changes in population density and composition as a function of cropping system and time. Our initial efforts have focused on: 1) developing high sample capacity nucleic acid extraction techniques that minimize the humic substance contamination, 2) assessing the reproducibility of [...] More »

Preliminary Corn Root Health Studies

Abstract: Roots were sampled from corn plants following corn (Rotation 1) and corn following red clover/wheat (Rotation 3) at the Arlington Research Station and Lakeland Agricultural Complex. These samples were analyzed for root discoloration, root density, and colonization by Pythium. Roots from the June 9 sampling at Lakeland did not differ in severity of [...] More »